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JoVE Journal Bioengineering
Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
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Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro

Article DOI: 10.3791/60544-v • 11:21 min • February 16th, 2020
February 16th, 2020 •
1, 1,2
1William G. Lowrie Department of Chemical and Biomolecular Engineering, The Ohio State University, 2Comprehensive Cancer Center, The Ohio State University

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Summary
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This protocol generates bioparticle microarrays that provide spatially controlled neutrophil swarming. It provides easy access to the mediators that neutrophils release during migration and allows for quantitative imaging analysis.

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Bioparticle Microarrays Chemotactic Analysis Molecular Analysis Human Neutrophil Swarming In Vitro Analysis Cytokines Lipid Mediators Quantitative Imaging Analysis White Blood Cells Monocytes T Cells Cationic Polyelectrolite CP Solution Fluorescent Labeling Master Silicon Wafer Photolithography Procedures Rectangular Array Negative Photoresist UV Light Exposure
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