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Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Bioparticle Microarrays for Chemotactic and Molecular Analysis of Human Neutrophil Swarming in vitro
DOI:

11:21 min

February 16, 2020

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Chapters

  • 00:04Introduction
  • 00:37Microfabrication of Bioparticle Microarray
  • 06:14Sample Preparation and Assay
  • 07:32Supernatant Collection
  • 08:40Results: Neutrophil Swarming Around Bioparticle Clusters
  • 10:45Conclusion

Summary

Automatic Translation

This protocol generates bioparticle microarrays that provide spatially controlled neutrophil swarming. It provides easy access to the mediators that neutrophils release during migration and allows for quantitative imaging analysis.

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