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Chapters
- 00:04Introduction
- 00:37Transgenic Rat Generation
- 02:11Lentiviral Vector Microinjection
- 03:51Injected Embryo Transfer
- 05:50Vasectomy
- 07:02Results: Representative Embryo Survival Rates and Implantation Efficiency
- 08:30Conclusion
This article aims to provide the methodology for lentiviral transgenesis in rat embryos using multiple injections of a virus suspension into the zygote perivitelline space. Female rats that are mated with a fertile male strain with a different dominant fur color is used to generate pseudopregnant foster mothers.
