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Generation of Transgenic Rats using a Lentiviral Vector Approach
JoVE Journal
Bioengineering
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JoVE Journal Bioengineering
Generation of Transgenic Rats using a Lentiviral Vector Approach
DOI:

09:07 min

May 17, 2020

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Chapters

  • 00:04Introduction
  • 00:37Transgenic Rat Generation
  • 02:11Lentiviral Vector Microinjection
  • 03:51Injected Embryo Transfer
  • 05:50Vasectomy
  • 07:02Results: Representative Embryo Survival Rates and Implantation Efficiency
  • 08:30Conclusion

Summary

Automatic Translation

This article aims to provide the methodology for lentiviral transgenesis in rat embryos using multiple injections of a virus suspension into the zygote perivitelline space. Female rats that are mated with a fertile male strain with a different dominant fur color is used to generate pseudopregnant foster mothers.

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