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Journal / Bioengineering / Isolering av vuxna humana dermal fibroblaster…
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Bioengineering

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JoVE Journal Bioengineering
Isolering av vuxna humana dermal fibroblaster från buk hud och generering av inducerade pluripotenta stamceller med en icke-integrerande metod
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Isolering av vuxna humana dermal fibroblaster från buk hud och generering av inducerade pluripotenta stamceller med en icke-integrerande metod

Article DOI: 10.3791/60629-v • 10:52 min • January 19th, 2020
January 19th, 2020 •
*1, *1, 1, 1, 1, 1, 1, 1
1Department of Public Health, University of Naples Federico II
* These authors contributed equally

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Cell omprogrammering kräver införandet av viktiga gener, som reglerar och bibehålla pluripotenta cell tillstånd. Det protokoll som beskrivs möjliggör bildandet av inducerade pluripotenta stamceller (iPSCs) kolonier från Human dermal fibroblaster utan viral/integrera metoder men med hjälp av icke-modifierade RNAs (NM-RNAs) kombinerat med immunförsvaret undvikande faktorer minska cellulära försvarsmekanismer.

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Bioteknik inducerade pluripotenta stamceller (iPSCs) cell omprogrammering dermal fibroblaster icke-modifierad RNAs NM-RNAs integration-fri metod cellkultur Human regenerativ medicin
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