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Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
JoVE Journal
Biology
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JoVE Journal Biology
Isolation and Characterization of Adult Cardiac Fibroblasts and Myofibroblasts
DOI:

10:45 min

March 12, 2020

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Chapters

  • 00:05Introduction
  • 00:52Heart Dissection
  • 01:56Enzymatic Dissociation of the Heart
  • 04:42Fibroblast Isolation by Differential Plating
  • 06:00Isolation of Fibroblasts Using Magnetic Beads
  • 08:24Results: Fibroblasts in Injured and Uninjured Mouse Hearts
  • 09:57Conclusion

Summary

Automatic Translation

Obtaining a pure population of fibroblasts is crucial to studying their role in wound repair and fibrosis. Described here is a detailed method to isolate fibroblasts and myofibroblasts from uninjured and injured mouse hearts followed by characterization of their purity and functionality by immunofluorescence, RTPCR, fluorescence-assisted cell sorting, and collagen gel contraction.

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