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Journal / Biochemistry / Valutazione dell'ossidazione cellulare…
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Biochemistry

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JoVE Journal Biochemistry
Valutazione dell'ossidazione cellulare utilizzando una proteina fluorescente verde sensibile al comparto subcellulare
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Valutazione dell'ossidazione cellulare utilizzando una proteina fluorescente verde sensibile al comparto subcellulare

Article DOI: 10.3791/61229-v • 06:10 min • June 18th, 2020
June 18th, 2020 •
1,2, 1, 1, 1
1Division of Radiation Health, Pharmaceutical Sciences, University of Arkansas for Medical Sciences, 2Department of Pharmaceutical Toxicology, Faculty of Pharmacy, Ege University

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Questo protocollo descrive la valutazione dello stato di redox subcellulare specifico del compartimento all'interno della cella. Una sonda fluorescente redox-sensibile consente una comoda analisi ratiometrica nelle cellule intatte.

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Biochimica numero 160 proteina fluorescente verde sensibile alla rifare (roGFP) roGFP citosolico rapporto cisteina/cistina imaging delle cellule vive citometria di flusso ossidazione/riduzione
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