In JoVE (3)
- Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke
- Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair
- In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity
Other Publications (1)
Articles by Wei Zheng in JoVE
Rose Bengal Photothrombosis by Confocal Optical Imaging In Vivo: A Model of Single Vessel Stroke Lora Talley Watts1, Wei Zheng1, R. Justin Garling2, Victoria C. Frohlich3, James Donald Lechleiter1 1Department of Cellular and Structural Biology, The University of Texas Health Science Center San Antonio, 2School of Medicine, The University of Texas Health Science Center San Antonio, 3Cell & Tissue Imaging Center, St. Jude Childrens Research Hospital Here, we describe a semi-invasive optical microscopy approach for the induction of a Rose Bengal photothrombotic clot in the somatosensory cortex of a mouse in vivo. The technical aspects of the imaging procedure are described from induction of a photothrombotic event to application and data collection.
Visualizing Angiogenesis by Multiphoton Microscopy In Vivo in Genetically Modified 3D-PLGA/nHAp Scaffold for Calvarial Critical Bone Defect Repair Jian Li1, Holger Jahr2,3, Wei Zheng4, Pei-Gen Ren1 1Center for Translational Medicine Research and Development, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences, 2Department of Orthopedic Surgery, Maastricht UMC+, 3Department of Orthopaedic Surgery, University Hospital RWTH, 4Research Laboratory for Biomedical Optics and Molecular Imaging, Shenzhen Institutes of Advanced Technology, Chinese Academy of Sciences Here, we present a protocol to visualize blood vessel formation in vivo and in real-time in 3D scaffolds by multiphoton microscopy. Angiogenesis in genetically modified scaffolds was studied in a murine calvarial critical bone defect model. More new blood vessels were detected in the treatment group than in controls.
In Vitro Growth of Mouse Preantral Follicles Under Simulated Microgravity Shen Zhang1, Yonggen Wu1, Yimin Weng2, Zhihui Xu1, Wenmin Chen3, Dahan Zheng4, Wei Lin5, Jun Liu6, Ying Zhou1,7 1Reproductive Medicine Center, The First Affiliated Hospital of Wenzhou Medical University, 2Department of Orthopaedics, The Second Affiliated Hospital of Wenzhou Medical University, 3Department of Obstetrics, The First Affiliated Hospital of Wenzhou Medical University, 4School of Laboratory Medicine and Life Science, Wenzhou Medical University, 5School of Pharmaceutical Science, Wenzhou Medical University, 6Stem Cells and Genetic Engineering Group, AgriBioscience Research Centre, Department of Economic Development, Jobs, Transport and Resources, 7Department of Histology and Embryology, Wenzhou Medical University A highly promising technique to generate tissue constructs without using matrix is to culture cells in a simulated microgravity condition. Using a rotary culture system, we examined ovarian follicle growth and oocyte maturation in terms of follicle survival, morphology, growth, and oocyte function under the simulated microgravity condition.
Other articles by Wei Zheng on PubMed
Two Novel Ansamitocin Analogs from Actinosynnema Pretiosum Natural Product Research. 2013 | Pubmed ID: 23061718 By using various column chromatography for purification, two new compounds of ansamitocin (1 and 2) were isolated from the extracts of fermentation broth of Actinosynnema pretiosum FIM06-0063. Their structures were established as maytansinol-9-methyl ether-3-propionate (1) and maytansinol-9-methyl ether-3-3'-methy-butyrate (2), respectively, by an extensive NMR analysis.