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Bimolecular Fluorescence Complementation (BiFC) Assay for Protein-Protein Interaction in Onion Cells Using the Helios Gene Gun

Article DOI: 10.3791/1963-v • 10:39 min • June 12th, 2010
June 12th, 2010 •
1, 1
1Dept. Of Cell Biology and Molecular Genetics, University of Maryland

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This article illustrates how to properly use the BioRad Helios Gene Gun to introduce plasmid DNA into onion epidermal cells and how to test for protein-protein interactions in onion cells based on the principle of Bimolecular Fluorescence Complementation (BiFC)

Tags

Bimolecular Fluorescence Complementation BiFC Assay Protein-protein Interaction Onion Cells Helios Gene Gun Gene Function Fluorescent Protein GFP Plant Researchers Onion Epidermal Cells Plasmid DNA Arabidopsis Thaliana SEUSS LEUNIG HOMOLOG
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