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Bioengineering

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JoVE Journal Bioengineering
Bruke Microfluidics Chips for Live Imaging and Study of Injury Responses i
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Bruke Microfluidics Chips for Live Imaging and Study of Injury Responses i

Article DOI: 10.3791/50998-v • 11:46 min • February 7th, 2014
February 7th, 2014 •
1, 2, 1, 1, 1, 3,4, 2,5, 1
1Department of Molecular, Cellular and Developmental Biology, University of Michigan, 2Department of Biomedical Engineering, University of Michigan, 3Life Sciences Institute, University of Michigan, 4Department of Cell and Developmental Biology, University of Michigan, 5Department of Mechanical Engineering, University of Michigan

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Drosophila larver er et attraktivt modellsystem for levende avbildning på grunn av deres gjennomskinnelig skjellaget og kraftige genetikk. Denne protokollen beskriver hvordan du kan utnytte en single-layer PDMS enhet, kalt 'larve chip "for levende avbildning av cellulære prosesser i nevroner av 3 rd instar Drosophila larver.

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Bioteknologi , Live Imaging Microfluidics nerveskaden aksonal degenerasjon kalsium bildebehandling photoconversion laser mikrokirurgi
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