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JoVE Journal Genetics
Snelle verwijdering Productie in Fungi via
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Journal (Genetics)
Single-cell Gene Expression Using Multiplex RT-qPCR to Characterize Heterogeneity of Rare Lymphoid Populations
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
Journal (Genetics)
Determination of the Optimal Chromosomal Location(s) for a DNA Element in Escherichia coli Using a Novel Transposon-mediated Approach
 
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Snelle verwijdering Productie in Fungi via

Article DOI: 10.3791/55239-v • 11:24 min • June 12th, 2017
June 12th, 2017 •
1, 2, 3, 1
1Toxicology and Mycotoxin Research Unit, NPRC, USDA-ARS, 2Hazera Seeds LTD, Brurim, 3Instituto de Hortofruticultura Subtropical y Mediterránea “La Mayora” - Universidad de Málaga - Consejo Superior de Investigaciones Científicas (IHSM-UMA-CSIC), Estación Experimental “La Mayora”

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Gen deletie mutanten gegenereerd door homologe recombinatie zijn de gouden standaard voor genfunctie studies. De OSCAR (One Step Construction of Agrobacterium-Recombination-ready-plasmids) methode voor snelle generatie van deletieconstructen wordt beschreven. Agrobacterium gemedieerde schimmel transformatie volgt. Tenslotte wordt een PCR-gebaseerde bevestigingsmethode van gendeleties in zwamtransformanten gepresenteerd.

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Genetica Uitgave 124 Schimmelvorming Schimmelvorming mutant generatie ATMT One Step Construction of
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