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JoVE Journal Neuroscience
Modelleren van neuronale dood en degeneratie in muis primaire cerebellaire submodule neuronen
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Modelleren van neuronale dood en degeneratie in muis primaire cerebellaire submodule neuronen

Article DOI: 10.3791/55871-v • 10:36 min • November 6th, 2017
November 6th, 2017 •
1,2, 1, 3, 1,2,4
1Lady Davis Institute for Medical Research, Jewish General Hospital, 2Integrated Program in Neuroscience, McGill University, 3Department of Cellular and Molecular Medicine, University of Ottawa, 4Department of Oncology, Faculty of Medicine, McGill University

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Dit protocol beschrijft een eenvoudige methode om te isoleren en primaire muisknop cerebrale submodule neuronen (CGNs) van 6-7 dag oud pups, efficiënte transductie van CGNs voor verlies en winst van functie studies, kweken en modellering NMDA-geïnduceerde neuronale excitotoxicity, lage-kalium-veroorzaakte celdood, DNA-schade en oxidatieve stress met behulp van de zelfde cultuur-model.

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Neurowetenschappen kwestie 129 Cerebellum cerebellaire submodule neuronen (CGN) Adenovirus lentivirale signaaltransductie Excitotoxicity N-methyl-D-aspartaat (NMDA) Apoptosis kaliumchloride (KCl) camptothecine waterstof peroxide (H2O2)
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