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Journal / Immunology and Infection / Generere rekombinant Avian Herpesvirus vektorer…
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Immunology and Infection

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Generere rekombinant Avian Herpesvirus vektorer med CRISPR/Cas9 Gene redigering

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvirus av kalkuner (HVT) er mye brukt som en vektor plattform for generering av rekombinant vaksiner mot en rekke avian sykdommer. Denne artikkelen beskriver en enkel og rask tilnærming for generering av rekombinant HVT-vectored vaksiner med en integrert NHEJ-CRISPR/Cas9 og grobunn-Lox system.

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Immunologi og infeksjon problemet 143 CRISPR/Cas9 NHEJ grobunn-LoxP HVT rekombinante vaksine avian sykdommer
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