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Journal / Immunology and Infection / Geração de vetores recombinantes Herpesvirus…
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Immunology and Infection

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JoVE Journal Immunology and Infection
Geração de vetores recombinantes Herpesvirus aviária com CRISPR/Cas9 Gene edição
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Geração de vetores recombinantes Herpesvirus aviária com CRISPR/Cas9 Gene edição

Article DOI: 10.3791/58193-v • 12:21 min • January 7th, 2019
January 7th, 2019 •
1,2, 1, 1, 1, 1, 1, 2, 1, 1
1The Pirbright Institute & UK-China Centre of Excellence for Research on Avian Diseases, 2Binzhou Animal Science and Veterinary Medicine Academy & UK-China Centre of Excellence for Research on Avian Diseases

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Herpesvírus de perus (HVT) é amplamente utilizado como uma plataforma de vetor para a geração de vacinas recombinantes contra um número das doenças aviárias. Este artigo descreve uma abordagem simples e rápida para a geração de vacinas recombinantes HVT-vectored, usando um sistema de Cre-Lox e integrado NHEJ-CRISPR/Cas9.

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Imunologia e infecção edição 143 CRISPR/Cas9 NHEJ Cre-LoxP HVT vacina recombinante as doenças aviárias
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