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Journal / Immunology and Infection / 在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究…
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Immunology and Infection

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JoVE Journal Immunology and Infection
在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究
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在细胞堆中生产腺相关病毒载体，用于大型动物模型的临床前研究

Article DOI: 10.3791/62727-v • 07:21 min • June 30th, 2021
June 30th, 2021 •
1, *1, *1, *1, 1, 1, 1, 1, 1
1Department of Pathobiology, University of Guelph
* These authors contributed equally

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在这里，我们提供了使用在细胞堆叠中生长的贴壁HEK 293细胞和亲和色谱纯化大规模生产研究级AAV载体的详细程序。该方案始终如一地产生>1 x 1013 个载体基因组/ mL，提供适合大型动物研究的载体量。

Tags

免疫学和感染，第172期，腺相关病毒载体，基因治疗，细胞堆栈，亲和纯化，临床前研究，大型动物模型，转基因表达，AAV6，AAV6.2FF
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