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JoVE Journal Developmental Biology
Isolering av råtta fettvävnad mesenkymala stamceller för differentiering till insulinproducerande celler
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Isolering av råtta fettvävnad mesenkymala stamceller för differentiering till insulinproducerande celler

Article DOI: 10.3791/63348-v • 08:09 min • August 29th, 2022
August 29th, 2022 •
1, 2, 2, 1,2,3
1Biochemistry Department, Faculty of Pharmacy, Ain Shams University, 2Pharmacology and Biochemistry Department, Faculty of Pharmacy, The British University in Egypt, 3Center for Drug Research and Development (CDRD), Faculty of Pharmacy, The British University in Egypt

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Fettvävnads-härledda mesenkymala stamceller (Ad-MSC) kan vara en potentiell källa till MSC som differentieras till insulinproducerande celler (IPC). I detta protokoll tillhandahåller vi detaljerade steg för isolering och karakterisering av råtta epididymala Ad-MSC, följt av ett enkelt, kort protokoll för generering av IPC från samma råtta Ad-MSCs.

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Utvecklingsbiologi nummer 186 Stamceller mesenkymala stamceller diabetes insulinproducerande celler fettvävnad differentiering
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