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Generation of Bone Marrow Derived Murine Dendritic Cells for Use in 2-photon Imaging

Article DOI: 10.3791/773-v • 13:13 min • July 9th, 2008
July 9th, 2008 •
1, 1, 1, 2
1Department of Physiology and Biophysics, University of California, Irvine (UCI), 2Department of Neurobiology and Behaviour, University of California, Irvine (UCI)

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Antigen presentation in secondary lymphoid organs by dendritic cells is crucial for the initiation of the T cell mediated adaptive immune response. Here we demonstrate the culture of bone marrow derived murine dendritic cells, activation, and labeling for 2-photon imaging.

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Bone Marrow Derived Murine Dendritic Cells 2-photon Imaging Preparation Method CD11c High Dendritic Cells Culture Homing Draining Lymph Node Antigen Presentation T Cell Interaction Essen Instruments Incucyte Dendritic Cell Maturation Peripheral Lymph Nodes Antigen-specific Interactions Two-photon Imaging
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