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Journal / Biology / のタンパク質複合体の同定
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JoVE Journal Biology
のタンパク質複合体の同定
Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
Journal (Biology)
Use of MALDI-TOF Mass Spectrometry and a Custom Database to Characterize Bacteria Indigenous to a Unique Cave Environment (Kartchner Caverns, AZ, USA)
Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
Journal (Biology)
Protocol for Culturing Sympathetic Neurons from Rat Superior Cervical Ganglia (SCG)
 
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のタンパク質複合体の同定

Article DOI: 10.3791/4057-v • 14:58 min • November 12th, 2012
November 12th, 2012 •
1,2, 1, 1, 1,3, 1,3
1Banting and Best Department of Medical Research, Donnelly Centre, University of Toronto, 2Deparment of Biochemistry, Research and Innovation Centre, University of Regina, 3Department of Medical Genetics and Microbiology, University of Toronto

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マススペクトロメトリー（APMS）との組み合わせでタグ付けされたタンパク質のアフィニティー精製は、タンパク質相互作用ネットワークの体系的マッピングのため、生物学的プロセスの基本メカニズムを調査するための強力な方法です。ここでは、細菌のために開発最適化された順次ペプチドアフィニティー（SPA）をAPMSの手順を説明

Tags

遺伝学、問題69、分子生物学、医学、生化学、微生物学、アフィニティー精製、
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