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Biochemistry

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JoVE Journal Biochemistry
Undersøgelse af funktioner og aktiviteter af neuronal K-Cl Co-Transporter KCC2 ved hjælp af Western Blotting
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Undersøgelse af funktioner og aktiviteter af neuronal K-Cl Co-Transporter KCC2 ved hjælp af Western Blotting

Article DOI: 10.3791/64179-v • 10:08 min • December 9th, 2022
December 9th, 2022 •
1, 1, 1, 1
1Institute of Biomedical and Clinical Sciences, Medical School, Faculty of Health and Life Sciences, University of Exeter, Hatherly Laboratories

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Den nuværende protokol fremhæver anvendelsen af western blotting teknik til at studere funktioner og aktiviteter af neuronal K-Cl co-transporter KCC2. Protokollen beskriver undersøgelsen af KCC2-phosphorylering på kinaseregulerende steder Thr906/1007 via western blotting. Yderligere metoder til bekræftelse af KCC2-aktivitet er også kort fremhævet i denne tekst.

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