Farelerde floresan haberci kullanılarak, akciğer metastazı olan tümör hücre stromanın etkileşimleri ex vivo canlı görüntüleme için nispeten basit bir yöntem tarif eder. iplik disk konfokal mikroskopi kullanılarak, bu teknik, en az 4 saat boyunca canlı hücre görüntülenmesini sağlayarak ve diğer enflamatuar akciğer koşulları incelemek için adapte edilebilir.
Metastaz kanserle ilgili morbidite ve mortalite açısından önemli bir nedenidir. Metastaz çok aşamalı bir süreçtir ve karmaşıklığı nedeniyle, metastatik yayılması ve büyümesi yöneten tam hücresel ve moleküler süreçler hala sürüncemede bulunmaktadır. Canlı görüntüleme hücreleri ve bunların mikro dinamik ve mekansal etkileşimlerin görselleştirme sağlar. Solid tümörler genellikle akciğerlere metastaz. Ancak, akciğerlerde anatomik konumu intravital görüntüleme bir sorun teşkil etmektedir. Bu protokol, tümör hücreleri ve akciğer metastazı içindeki çevreleyen stroma arasındaki dinamik etkileşimin ex vivo canlı görüntüleme için nispeten basit ve hızlı bir yöntemdir. Bu yöntemi kullanarak, kendi mikro-kanser hücrelerinin stromal hücreleri arasında kanser hücrelerinin hareketliliği ve etkileşimler birkaç saat gerçek zamanlı olarak görülebilir. Transgenik flüoresan raportör fareler kullanılarak, bir flüoresan hücre çizgisi, enjekte edilebilir floresan etiketlimoleküller ve / veya antikorlar, akciğer mikro birden fazla bileşenler, kan damarları ve bağışıklık hücreleri gibi, görselleştirilebilir. farklı hücre tipleri görüntü için, hızlı, dört renkli görüntü elde uzun vadeli, sürekli görüntüleme sağlar dönen bir disk konfokal mikroskop kullanılmıştır. Birden fazla pozisyonları ve odak düzlemleri boyunca toplanan görüntülerden derlenen Time-lapse film en az 4 saat süreyle canlı metastatik ve bağışıklık hücreleri arasındaki etkileşimleri göstermektedir. Bu teknik, ayrıca, kemoterapi veya hedeflenen tedavi test etmek için de kullanılabilir. Dahası, bu yöntem, akciğer mikro-etkileyebilecek diğer akciğer ile ilgili patolojilerin çalışma için uyarlanabilir.
The deadliest aspect of cancer is metastasis, which accounts for more than 90% of cancer-related morbidity and mortality1. Metastasis is a multistep process and due to its complexity, the exact cellular and molecular mechanisms that govern metastatic dissemination and growth are still elusive. To metastasize, tumor cells in the primary tumor must detach from their neighboring cells and basement membrane, cross through the extracellular matrix, intravasate, travel via blood or lymphatic vessels, extravasate at the secondary site, and finally, survive and establish secondary tumors. In addition to the properties of the tumor cells, the contribution from the microenvironment, which includes the adjacent stroma along with the normal counterparts of the cancer cells, is crucial for the seeding and establishment of metastatic lesions2.
Traditional methods to study metastatic seeding and growth examine static states, as tissues are excised and sectioned for histology. These data only generate a snapshot of this highly dynamic process. Although some useful information can be gained from these studies, the complicated process by which tumor and stromal cells interact during metastatic formation cannot be adequately assessed by these methods. Furthermore, it is not possible to gain insights into tumor or stromal cell migration patterns, which are important in establishing a colony at the distant site. In order to effectively study the metastatic process, it is essential to visualize various interactions between cancer cells and their microenvironment in a continuous manner and at real time.
The lung is a common site for metastases from solid tumors as breast, colorectal, pancreatic cancer, melanoma and sarcoma3. Intravital imaging was previously used to study cell-cell interaction in various primary tumor and metastatic models4,5. Methods of lung imaging in mice, including intravital imaging, lung section imaging, and an ex vivo pulmonary metastasis assay have been published6–9. Intravital imaging of mouse lungs utilizes a thoracic suction window to stabilize the lungs6. This method is used for time-lapse imaging of the lung microcirculation and alveolar spaces. The anatomical location of the lungs poses a challenge to intravital imaging. In order to access the lungs, the chest cavity must be opened which leads to loss of negative pressure and collapsed lungs. This method only allows the visualization of a small part of the lungs and is technically demanding; an unnecessary complication in studies that examine processes that are independent of blood flow. Moreover, this method also requires gating out movement caused by breathing. This is done either by collecting images between breaths or during post image acquisition analyses10. The alternative ex vivo lung section imaging provides stability and depth, and also prepares lung parenchyma for immunostaining7. However, the lengthy sectioning process leads to an extensive delay between the time of animal sacrifice and the start of the imaging session. Moreover, the process of sectioning a mouse lung causes considerable amount of cell death8, thus interfering with the quality and quantity of imaging samples and perhaps needlessly altering tumor-stroma interactions. In order to technically bridge between the methods of intravital imaging and lung section imaging, while exploiting the advantages of the two techniques, a relatively fast and easy method for ex vivo lung imaging was developed. This method was achieved by imaging of non-sectioned whole lung lobes. Using this method, the motility of cancer cells as well as interactions between cancer cells and stromal cells in their microenvironment can be visualized in real time for several hours.
Bu yazıda metastaz taşıyan fare modellerinde akciğer metastazı ex vivo canlı görüntüleme için ayrıntılı bir yöntemi tarif etmektedir. Bu görüntüleme protokol akciğer mikroçevresinin içinde dinamik ve mekansal tümör hücre-stroma etkileşimleri doğrudan görselleştirme sağlar. En az 4 saat akciğer metastazı güvenilir bir görüntüleme sağlayan bir kolay ve hızlı bir yöntemdir. Bu deneylerden elde edilen filmler, hücre motilitesi ve hücresel etkileşimler gibi dinamik işlemleri…
The authors have nothing to disclose.
We thank Nguyen H. Nguyen for her technical help and Audrey O’Neill for support with the Zeiss Cell Observer spinning-disk confocal microscope. This work was supported by a Department of Defense postdoctoral fellowship (W81XWH-11-01-0139) and the Weizmann Institute of Science-National Postdoctoral Award Program for Advancing Women in Science (to V.P.).
MMTV-PyMT/FVB mice | Jackson Laboratory | 2374 | Female mice |
ACTB-ECFP/FVB mice | UCSF Werb lab | Female mice | |
c-fms-EGFP/FVB mice | UCSF Werb lab | Female mice | |
FVB mice | Jackson Laboratory | 1800 | Female mice |
GFP+ VO-PyMT cells | UCSF Werb lab | ||
70,000 kDa Dextran, rhodamine-conjugated | Invitrogen | D1818 | Dilute to 4mg/ml in 1 x PBS and store at -20 °C. Use 0.4 mg per animal. |
10,000 kDa Dextran, Alexa Fluor 647 conjugated | Invitrogen | D22914 | Dilute to 4mg/ml in 1 x PBS and store at -20 °C. Use 0.4 mg per animal. |
Anti-mouse Gr-1 antibody Alexa Fluor 647 | UCSF Monoclonal antibody core | Stock 1mg/ml. Use 7 ug per animal. | |
Anesthetic | Anesthesia approved by IACUC, used for anesthesia and/or euthanesia | ||
1X PBS | UCSF cell culture facility | ||
PBS, USP sterile | Amresco INC | K813-500ML | Ultra pure grade for i.v. injection |
Styrofoam platform | Will be used as dissection board | ||
Fine scissors sharp | Fine Science Tools | 14060-11 | |
Forceps | Roboz Surgical Store | RS-5135 | |
Hot bead sterilizer | Fine Science Tools | 18000-45 | Turn ON 30min before use |
Air | UCSF | ||
Oxygen | UCSF | ||
Carbon dioxide | UCSF | ||
1 mL syringe without needle | BD | 309659 | |
27 G x 1/2 needle | BD | 305109 | for i.v. injection |
20 G x 1 needle, short bevel | BD | 305178 | |
Low-melting-temperature agarose | Lonza | 50111 | To make 10 ml of solution, weigh 0.2 g of agarose, add to 10 ml 1 x PBS, and heat to dissolve. Agarose will solidify at room temperature, so maintain in a 37 °C water bath until used for inflation. |
RPMI-1640 medium without phenol red | Life Technologies | 11835-030 | |
24 well Imaging plate | E&K scientific | EK-42892 | |
Glass cover slides, 15 mm | Fisher Scientific | 22-031-144 | |
Digital CO2 and temperature controller | Okolab | DGTCO2BX | http://www.oko-lab.com |
Climate chamber | Okolab | http://www.oko-lab.com | |
Cell Observer spinning disk confocal microscope | Zeiss | ||
Zen software | Zeiss | ||
Inverted microscope | Carl Zeiss Inc | Zeiss Axiovert 200M | |
ICCD camera | Stanford Photonics | XR-Mega-10EX S-30 | |
Spinning disk confocal scan-head | Yokogawa Corporation | CSU-10b | |
Imaris | Bitplane | ||
mManager | Vale lab, UCSF | Open-source software |