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非洲爪蟾卵母细胞的微量注射
JoVE Journal
Biology
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JoVE Journal
Biology
Microinjection of Xenopus Laevis Oocytes
Please note that all translations are automatically generated.
Click here for the English version.
非洲爪蟾卵母细胞的微量注射
DOI:
10.3791/1106-v
•
12:10 min
•
February 23, 2009
•
Sarah Cohen
,
Shelly Au
,
Nelly Panté
1
Department of Zoology
,
University of British Columbia – UBC
Chapters
00:00
Title
01:04
Introduction
01:30
Preparation of Xenopus Oocytes for Microinjection
03:24
Microinjection of Xenopus Oocytes
05:54
Dissection of Xenopus Oocytes
07:03
Preparation of Injected Oocytes for Embedding and Thin-Sectioning EM
10:02
Representative Results/Outcome
11:24
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们展示了胞浆内显微注射的<em>非洲爪蟾</em>卵母细胞的核进口基材,以及准备注入卵母细胞超薄切片电子显微镜可视化。
Tags
Microinjection
Xenopus Laevis Oocytes
Electron Microscopy (EM)
Nucleocytoplasmic Transport
Nuclear Pore Complexes (NPCs)
Nuclear Import
Nuclear Export
Viruses
Host Nucleus
Cytoplasmic Microinjection
Nuclear Import Substrate
Thin-sectioning EM
Dissection
Dehydration
Embedding
Epoxy Embedding Resin
Baculovirus Autographa Californica Nucleopolyhedrovirus (AcMNPV)
Parvovirus Minute Virus Of Mice (MVM)
Technique
Article
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