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一个神经元和星形胶质细胞共培养检测神经毒性的高含量分析
JoVE Journal
Biology
This content is Free Access.
JoVE Journal Biology
A Neuronal and Astrocyte Co-Culture Assay for High Content Analysis of Neurotoxicity
DOI:

15:04 min

May 05, 2009

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Chapters

  • 00:05Title
  • 00:48Introduction
  • 01:28Cell Preparation for Neurotoxicity Screens
  • 02:57Cell Fixation and Immunofluorescent Staining
  • 06:07HCS Imaging, Analysis, and Results
  • 14:15Conclusion

Summary

Automatic Translation

本文介绍了一种新的协议和试剂,为敏感的神经毒性作用的化合物和治疗联合培养的神经元和星形胶质细胞使用高含量的分析测量设计。结果表明,高含量的分析,代表着一个令人兴奋的新技术,神经毒性评估。

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