JoVE
JoVE
Faculty Resource Center
Research
Behavior
Biochemistry
Biology
Bioengineering
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
JoVE Journal
JoVE Encyclopedia of Experiments
JoVE Chrome Extension
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to your Course
Authors
Librarians
High Schools
About
Sign-In
Sign In
Contact Us
Research
JoVE Journal
JoVE Encyclopedia of Experiments
Education
JoVE Core
JoVE Science Education
JoVE Lab Manual
High Schools
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Close
Research
Behavior
Biochemistry
Bioengineering
Biology
Cancer Research
Chemistry
Developmental Biology
Engineering
Environment
Genetics
Immunology and Infection
Medicine
Neuroscience
Products
JoVE Journal
JoVE Encyclopedia of Experiments
Education
Biology
Chemistry
Clinical
Engineering
Environmental Sciences
Pharmacology
Physics
Psychology
Statistics
Products
JoVE Core
JoVE Science Education
JoVE Lab Manual
JoVE Quiz
JoVE Business
Videos Mapped to Your Course
Teacher Resources
Get in Touch
Instant Trial
Log In
EN
EN - English
CN - 中文
DE - Deutsch
ES - Español
KR - 한국어
IT - Italiano
FR - Français
PT - Português
Journal
/
Biology
/
在芽殖酵母中的自我复制寿命测量
JoVE Journal
Biology
A subscription to JoVE is required to view this content.
Sign in or start your free trial.
JoVE Journal
Biology
Measuring Replicative Life Span in the Budding Yeast
Please note that all translations are automatically generated.
Click here for the English version.
在芽殖酵母中的自我复制寿命测量
DOI:
10.3791/1209-v
•
12:41 min
•
June 25, 2009
•
Kristan K. Steffen
,
Brian K. Kennedy
,
Matt Kaeberlein
1
Department of Biochemistry
,
University of Washington
,
2
Department of Pathology
,
University of Washington
Chapters
00:05
Title
00:26
Introduction
00:49
Prepare Strains and Plates for Replicative Life Span Analysis
03:35
Position Cells for Replicative Life Span Analysis
06:22
Obtain Virgin Daughter Cells for Life Span Analysis
08:37
Measuring the Replicative Capacity of Individual Cells
11:18
Representative Results
12:10
Conclusion
Summary
Automatic Translation
English (Original)
العربية (Arabic)
中文 (Chinese)
Nederlands (Dutch)
français (French)
Deutsch (German)
עברית (Hebrew)
italiano (Italian)
日本語 (Japanese)
한국어 (Korean)
português (Portuguese)
русский (Russian)
español (Spanish)
Türkçe (Turkish)
Automatic Translation
在这篇文章中,我们提出了一个用于测量酵母母细胞的复制寿命一般协议。
Tags
Replicative Life Span
Budding Yeast
Aging
Cellular Components
Organelles
Mortality
Saccharomyces Cerevisiae
Biology Of Aging
Age-associated Phenotypes
Life Span Paradigms
Chronological Life Span
Quiescence-like State
Post-mitotic Cells
Replicative Life Span Assay
Daughter Cells
Senescence
Mitotically Active Cells
Light Microscope
Article
Embed
ADD TO PLAYLIST
Usage Statistics
Related Videos
测量
线虫
固体介质的寿命
量化老化细胞生长的酵母年谱跨度
纯化
M。 magneticum
应变AMB - 1磁小体相关蛋白MamAΔ41
在芽殖酵母中的蛋白浓度的功能形态学表型的发展分析
测量
线虫
寿命在96孔板
测量单个活细胞中的mRNA转录的动力学
获取荧光时间推移电影的芽殖酵母和分析单细胞动力学移植物
芽殖酵母蛋白质的提取和纯化功能研究,交互和翻译后修饰
测量渗透水渗透系数(P<sub> F</sub>)球形细胞:隔离植物原生质体为例。
蛋白质纯化技术,可以检测SUMO化修饰和芽殖酵母蛋白动粒和Ndc10泛素Ndc80的
Read Article