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Biology
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蛋白泛素化的检测
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Biology
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JoVE Journal
Biology
Detection of Protein Ubiquitination
Please note that all translations are automatically generated.
Click here for the English version.
蛋白泛素化的检测
DOI:
10.3791/1293-v
•
09:00 min
•
August 19, 2009
•
Yeun Su Choo
,
Zhuohua Zhang
1
Signal Transduction Program
,
The Sanford Burnham Institute for Medical Research
Chapters
00:10
Introduction
00:42
Expression and Purification of Target Protein and Tagged Ubiquitin
03:15
Immunoprecipitation
04:51
SDS-PAGE and Immunostaining
05:48
Detection of Protein Ubiquitination in vitro
07:02
Representative Results
08:22
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
泛素化是一个关键的三种酶进行转录后修饰。在此修改有关的基因突变与许多不同的人类疾病。在这里,我们描述了协议检测培养细胞中的蛋白质的泛素<em>在体内</em>和试管<em>在体外</em>。
Tags
Protein Ubiquitination
Posttranslational Modification
Ubiquitin-activating Enzyme E1
Ubiquitin-conjugating Enzyme E2
Ubiquitin Ligase E3
Deubiquitylating Enzymes
Protein Stability
Cellular Localization
Biological Activity
Gene Mutations
Human Diseases
Cancer
Neurodegeneration
Metabolic Disorders
Pathogenesis
Detection Protocols
Cultured Cells In Vivo
Test Tubes In Vitro
Sumolyation
Neddylation
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