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蛋白泛素化的检测
JoVE Journal
Biology
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JoVE Journal Biology
Detection of Protein Ubiquitination
DOI:

09:00 min

August 19, 2009

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Chapters

  • 00:10Introduction
  • 00:42Expression and Purification of Target Protein and Tagged Ubiquitin
  • 03:15Immunoprecipitation
  • 04:51SDS-PAGE and Immunostaining
  • 05:48Detection of Protein Ubiquitination in vitro
  • 07:02Representative Results
  • 08:22Conclusion

Summary

Automatic Translation

泛素化是一个关键的三种酶进行转录后修饰。在此修改有关的基因突变与许多不同的人类疾病。在这里,我们描述了协议检测培养细胞中的蛋白质的泛素<em>在体内</em>和试管<em>在体外</em>。

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