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一个<em在体外皮肤刺激测试(SIT),使用的表皮重建人表皮(RHE)型号
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
An
In Vitro
Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
Please note that all translations are automatically generated.
Click here for the English version.
一个<em在体外皮肤刺激测试(SIT),使用的表皮重建人表皮(RHE)型号
DOI:
10.3791/1366-v
•
21:16 min
•
July 13, 2009
•
Helena Kandárová
,
Patrick Hayden
,
Mitchell Klausner
,
Joseph Kubilus
,
John Sheasgreen
1
MatTek Corporation
Chapters
00:05
Title
01:32
Introduction
02:07
The EpiDerm – Reconstructed Human Epidermal Model
04:02
Day 0 – Preparing for the Assay
05:21
Day 0 – Tissue Conditioning
08:09
Day 1 – Chemical Exposure
15:28
Day 2 – Media Change
16:03
Day 3 – MTT Viability Assay
19:55
Representative Results
20:33
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
在这段视频中,我们展示了开发和验证的表皮皮肤刺激性试验(表皮薛)<em>在体外</em>化学品的皮肤刺激测试,包括化妆品和药品的成分。
Tags
In Vitro Skin Irritation Test
EpiDerm Reconstructed Human Epidermal Model
EpiDerm SIT
In Vitro Skin Irritation Testing
Cosmetic Ingredients
Pharmaceutical Ingredients
GHS Category 2
MTT Viability Assay
Test Chemicals
Positive Control
Negative Control
60-minute Exposure
42-hour Post-incubation
Incubator
Cytokines
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