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An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model
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Biology
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JoVE Journal Biology
An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

An In Vitro Skin Irritation Test (SIT) using the EpiDerm Reconstructed Human Epidermal (RHE) Model

DOI:
10.3791/1366-v

•

21:16 min

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July 13, 2009

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, , , ,
1MatTek Corporation

Chapters

  • 00:05Title
  • 01:32Introduction
  • 02:07The EpiDerm – Reconstructed Human Epidermal Model
  • 04:02Day 0 – Preparing for the Assay
  • 05:21Day 0 – Tissue Conditioning
  • 08:09Day 1 – Chemical Exposure
  • 15:28Day 2 – Media Change
  • 16:03Day 3 – MTT Viability Assay
  • 19:55Representative Results
  • 20:33Conclusion

Summary

Automatic Translation

Automatic Translation

In this video, we demonstrate the EpiDerm Skin Irritation test (EpiDerm SIT) developed and validated for in vitro skin irritation testing of chemicals, including cosmetic and pharmaceutical ingredients.

Tags

In Vitro Skin Irritation TestEpiDerm Reconstructed Human Epidermal ModelEpiDerm SITIn Vitro Skin Irritation TestingCosmetic IngredientsPharmaceutical IngredientsGHS Category 2MTT Viability AssayTest ChemicalsPositive ControlNegative Control60-minute Exposure42-hour Post-incubationIncubatorCytokines

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