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大型插入环境基因组文库生产
JoVE Journal
Biology
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JoVE Journal
Biology
Large Insert Environmental Genomic Library Production
Please note that all translations are automatically generated.
Click here for the English version.
大型插入环境基因组文库生产
DOI:
10.3791/1387-v
•
20:59 min
•
September 23, 2009
•
Marcus Taupp
,
Sangwon Lee
,
Alyse Hawley
,
Jinshu Yang
,
Steven J. Hallam
1
Department of Microbiology and Immunology
,
University of British Columbia – UBC
Chapters
00:00
Title
00:07
Introduction
00:43
End Repair of Sheared Genomic DNA
02:10
Size-Selection of Genomic DNA by Pulsed-Field Gel Electrophoresis (PFGE)
04:48
DNA-recovery by Gel Extraction and Ligation of Recovered DNA to the Fosmid Cloning Vector
10:18
Phage-packaging of Vector-insert Ligation Product and Infection of Phage-Resistant Bacteria
14:30
Plating and Titering
16:34
Preparation for Colony Picking
17:46
Colony Picking, Culturing, and Long-Term Storage
20:32
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
是描述一个与环境基因组DNA经季节性缺氧峡湾的垂直深度连续分离的fosmid库建设。由此产生的克隆库回升到384孔板和存档,为下游的测序和一个自动化殖民地采摘系统的应用功能筛选。
Tags
Large Insert Environmental Genomic Library Production
Microbes
Cultivation Methods
Metagenomic Approaches
Genetic Content
Indigenous Microbial Communities
Genomic DNA Libraries
Cloning Techniques
Hypoxic Fjord
DNA Extraction And Purification Protocol
End-repaired DNA
Pulsed-field Gel Electrophoresis (PFGE)
Gel Extraction
DNA Fragments
Base Pairs (Kb)
Fosmid CopyControl Vector PCC1
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