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Chapters
- 00:00Title
- 00:07Introduction
- 00:43End Repair of Sheared Genomic DNA
- 02:10Size-Selection of Genomic DNA by Pulsed-Field Gel Electrophoresis (PFGE)
- 04:48DNA-recovery by Gel Extraction and Ligation of Recovered DNA to the Fosmid Cloning Vector
- 10:18Phage-packaging of Vector-insert Ligation Product and Infection of Phage-Resistant Bacteria
- 14:30Plating and Titering
- 16:34Preparation for Colony Picking
- 17:46Colony Picking, Culturing, and Long-Term Storage
- 20:32Conclusion
Mevsimsel hipoksik fiyort dikey derinliği süreklilik çevre genomik DNA izole bir fosmid kütüphane inşaatı açıklanmıştır. Çıkan klon kütüphanesi 384-kuyucuğu içine aldı ve mansap sıralama ve fonksiyonel tarama, otomatik bir koloni toplama sistemi uygulama tarafından arşivlenir.
Tags
Large Insert Environmental Genomic Library ProductionMicrobesCultivation MethodsMetagenomic ApproachesGenetic ContentIndigenous Microbial CommunitiesGenomic DNA LibrariesCloning TechniquesHypoxic FjordDNA Extraction And Purification ProtocolEnd-repaired DNAPulsed-field Gel Electrophoresis (PFGE)Gel ExtractionDNA FragmentsBase Pairs (Kb)Fosmid CopyControl Vector PCC1
