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变性尿素聚丙烯酰胺凝胶电泳(尿素页)
JoVE Journal
Biology
Author Produced
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JoVE Journal Biology
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
DOI:

08:44 min

October 29, 2009

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Chapters

  • 00:00Title
  • 00:08Introduction
  • 00:52Gel Preparation
  • 02:37Prerun
  • 04:03Load Samples and Run the Gel
  • 05:43Process the gel

Summary

Automatic Translation

变性尿素聚丙烯酰胺凝胶电泳用于分离单链DNA或RNA的500个核苷酸的限制。在热变性样品和非结构化的单链结合的尿素凝胶基质内迁移,根据其分子量。

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