Journal
/
Biology
/
变性尿素聚丙烯酰胺凝胶电泳（尿素页）
JoVE Journal
Biology
Author Produced
A subscription to JoVE is required to view this content.  Sign in or start your free trial.
JoVE Journal Biology
Denaturing Urea Polyacrylamide Gel Electrophoresis (Urea PAGE)
Please note that all translations are automatically generated. Click here for the English version.

变性尿素聚丙烯酰胺凝胶电泳（尿素页）

DOI:
10.3791/1485-v

•

08:44 min

•

October 29, 2009

•

, ,
1School of Biological Sciences,Nanyang Technological University, Singapore – NTU, 2Singapore-MIT Alliance for Reserach and Technology (SMART)

Chapters

  • 00:00Title
  • 00:08Introduction
  • 00:52Gel Preparation
  • 02:37Prerun
  • 04:03Load Samples and Run the Gel
  • 05:43Process the gel

Summary

Automatic Translation

Automatic Translation

变性尿素聚丙烯酰胺凝胶电泳用于分离单链DNA或RNA的500个核苷酸的限制。在热变性样品和非结构化的单链结合的尿素凝胶基质内迁移，根据其分子量。

Tags

Denaturing Urea Polyacrylamide Gel ElectrophoresisUrea PAGEDNA SeparationRNA SeparationPolyacrylamide Gel MatrixMolecular Weight SeparationAcrylamide ConcentrationLower Molecular Weight FragmentsUnstructured DNA Or RNA MoleculesSingle Stranded DNASingle Stranded RNAOligonucleotidesEnzymatic Cleavage ReactionsGel PreparationGel Running

Article

Embed

ADD TO PLAYLIST

Usage Statistics

Related Videos

变性梯度凝胶电泳（DGGE）

优化的纤维蛋白凝胶珠检测血管生成的研究

使用琼脂糖凝胶为基础的微流体装置的评估神经干细胞的运动

半变性洗涤剂琼脂糖凝胶电泳筛选淀粉样蛋白聚合

快速，灵敏的胶体考马斯亮G - 250染色蛋白聚丙烯酰胺凝胶

蓝原生聚丙烯酰胺凝胶电泳（BN - PAGE）分析，从细胞裂解液多蛋白复合物

浇注和运行的一种蛋白质凝胶重用商​​业磁带

代谢途径的确认和发现通过 13 C - Proteinogenic氨基酸的标签

琼脂糖凝胶电泳分离DNA片段

DNA交联聚丙烯酰胺水凝胶的制备

Read Article