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诱导膜电压测量与DI - 8 - ANEPPS
JoVE Journal
Biology
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JoVE Journal Biology
Measuring the Induced Membrane Voltage with Di-8-ANEPPS
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诱导膜电压测量与DI - 8 - ANEPPS

DOI:
10.3791/1659-v

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05:52 min

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November 19, 2009

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, ,
1Department of Biomedical Engineering, Faculty of Electrical Engineering,University of Ljubljana

Chapters

  • 00:06Introduction
  • 01:49Preliminary Steps
  • 02:28Loading the cells with di-8-ANEPPS
  • 03:21Experiment and image acquisition
  • 04:35Image processing and analysis

Summary

Automatic Translation

Automatic Translation

外加电场诱导细胞膜上的电压，称为诱导膜电压（ΔΦ）。通过电位染料DI - 8 - ANEPPS，这是可能的非侵入性来衡量的ΔΦ。该视频显示测量的协议ΔΦ使用DI - 8 - ANEPPS。

Tags

Induced Membrane VoltageDi-8-ANEPPSCellElectric FieldCharge RedistributionCell MembraneVoltageInduced Transmembrane VoltageInduced Transmembrane Potential DifferenceDelta PhiResting VoltagePotentiometric Fluorescent DyesVariationsNoninvasivelyLipid BilayerFluorescence IntensityProtocolCell ShapeAmplitudeSpatial Distribution

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