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实时成像果蝇胚胎血细胞迁移
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Live Imaging Of Drosophila melanogaster Embryonic Hemocyte Migrations
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实时成像果蝇胚胎血细胞迁移

DOI:
10.3791/1696-v

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08:35 min

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February 12, 2010

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, , ,
1Department of Biology and Biochemistry,University of Bath, 2Randall Division of Cell and Molecular Biophysics,King’s College London

Chapters

  • 00:00Title
  • 01:06Introduction
  • 01:43Preparation
  • 02:50Procedure for Mounting Drosophila Embryos
  • 07:03Representative Results
  • 07:55Conclusion

Summary

Automatic Translation

Automatic Translation

果蝇血细胞分散在整个胚胎发育。此协议演示如何安装和图像这些用荧光标记的血细胞的胚胎的迁移。

Tags

Live ImagingDrosophila MelanogasterEmbryonic Hemocyte MigrationsIn Vitro MethodsPhysiologically Relevant EnvironmentMounting ProtocolFluorescently Labeled HemocytesGal4-uas SystemGenetically Encoded MarkersDevelopmental DispersalEmbryo CollectionOuter Chorion RemovalHalocarbon OilHydrophobic MembraneGlass CoverslipGross Migratory ParametersSpeedDirectionalityHigher Resolution ImagingF-actinMicrotubulesCytoskeletal Components

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