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Journal
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Biology
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实时成像
果蝇
胚胎血细胞迁移
JoVE Journal
Biology
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JoVE Journal
Biology
Live Imaging Of
Drosophila melanogaster
Embryonic Hemocyte Migrations
Please note that all translations are automatically generated.
Click here for the English version.
实时成像
果蝇
胚胎血细胞迁移
DOI:
10.3791/1696-v
•
08:35 min
•
February 12, 2010
•
Iwan R. Evans
,
Jennifer Zanet
,
Will Wood
,
Brian M. Stramer
1
Department of Biology and Biochemistry
,
University of Bath
,
2
Randall Division of Cell and Molecular Biophysics
,
King’s College London
Chapters
00:00
Title
01:06
Introduction
01:43
Preparation
02:50
Procedure for Mounting Drosophila Embryos
07:03
Representative Results
07:55
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
果蝇
血细胞分散在整个胚胎发育。此协议演示如何安装和图像这些用荧光标记的血细胞的胚胎的迁移。
Tags
Live Imaging
Drosophila Melanogaster
Embryonic Hemocyte Migrations
In Vitro Methods
Physiologically Relevant Environment
Mounting Protocol
Fluorescently Labeled Hemocytes
Gal4-uas System
Genetically Encoded Markers
Developmental Dispersal
Embryo Collection
Outer Chorion Removal
Halocarbon Oil
Hydrophobic Membrane
Glass Coverslip
Gross Migratory Parameters
Speed
Directionality
Higher Resolution Imaging
F-actin
Microtubules
Cytoskeletal Components
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