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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species
 
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An Improved Method of RNA Isolation from Loblolly Pine (P. taeda L.) and Other Conifer Species

Article DOI: 10.3791/1751-v 08:25 min February 22nd, 2010
February 22nd, 2010

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Summary

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Click here for the English version.

Many plant tissues, including phloem and xylem from loblolly pine (Pinus taeda L.), contain high levels of phenolics and polysaccharides that interfere with RNA purification. This presentation discusses techniques for the harvest of field-grown tissues and isolation of RNA of sufficient quality for microarrays and other genomic analyses.

Tags

RNA Isolation Conifer Species Loblolly Pine Pinaceae Family Phenolic Compounds Polysaccharides Rigorous Tissue Collection Procedures RNA Purification Organic Extraction Steps Microarray Analysis Genomic Analysis Field-collected Samples RNA Degradation Total RNA Yields Woody Tissues Oxidizing Agents Contaminants
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