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原位
贴壁和非贴壁的哺乳动物细胞的亚细胞分馏
JoVE Journal
Biology
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JoVE Journal
Biology
In situ
Subcellular Fractionation of Adherent and Non-adherent Mammalian Cells
Please note that all translations are automatically generated.
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原位
贴壁和非贴壁的哺乳动物细胞的亚细胞分馏
DOI:
10.3791/1958-v
•
09:20 min
•
July 23, 2010
•
Anyaporn Sawasdichai
,
Hsin-Tien Chen
,
Nazefah Abdul Hamid
,
Padma-Sheela Jayaraman
,
Kevin Gaston
1
Department of Biochemistry, School of Medical Sciences
,
University of Bristol
,
2
Division of Immunity and Infection, School of Medicine
,
University of Birmingham
Chapters
00:04
Title
00:48
Introduction
01:21
Attaching Cells to Coverslips
02:51
Subcellular Fractionation
06:23
Preparing Cells for Immunofluorescence Microscopy
08:15
Results of Subcellular Fractionation
08:57
Conclusion
Summary
Automatic Translation
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Automatic Translation
允许
在原位
显微镜盖玻片上的哺乳动物细胞的亚细胞分馏蛋白定位可视化。
Tags
In Situ Subcellular Fractionation
Adherent Mammalian Cells
Non-adherent Mammalian Cells
Protein Localization
Protein Compartmentalization
Protein Sub-populations
Specific Structures
Transcription Factors
Chromatin Association
Nuclear Scaffold
Nuclear Matrix
Microscope Coverslips
Protein Visualization
Antibodies
Fluorescent Fusion Proteins
Fluorescence Microscopy
Specific Compartments Or Structures
Protein Mapping
Western Blotting
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