JoVE Journal
Biology
Biology
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Chapters
Summary
Molecular shuttles consisting of functionalized microtubules gliding on surface-adhered kinesin motor proteins can serve as a nanoscale transport system. Here, the assembly of a typical shuttle system is described.
Tags
Cargo Loading Kinesin Molecular Shuttles Microtubules Intracellular Transport ATP Molecules Synthetic Environments Biotin Streptavidin Motility Assay Casein Tubulin Rhodamine-labeled Streptavidin
PLAYLIST
Microtubule Structure and Organization
Microtubule Dynamics
Regulation of Microtubule Structure and Dynamics
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Research • BiologyHigh-resolution Imaging and Analysis of Individual Astral Microtubule Dynamics in Budding Yeast -
Research • Cancer ResearchLive Imaging to Study Microtubule Dynamic Instability in Taxane-resistant Breast Cancers -
Research • Encyclopedia of Experiments: Cancer ResearchMicrotubule Binding Assay in CRC Cells: A Method to Examine Microtubule Binding Tau Protein in Colorectal Cancer Cells
Kinesins and Dyneins: Microtubule-Based Motor Proteins
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Research • EngineeringAssembling Molecular Shuttles Powered by Reversibly Attached Kinesins -
Research • BiologyCargo Loading onto Kinesin Powered Molecular Shuttles -
Research • BiochemistryProduction of Dynein and Kinesin Motor Ensembles on DNA Origami Nanostructures for Single Molecule Observation
Cilia and Flagella: Microtubule-Based Surface Structures
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Research • NeuroscienceObservation of the Ciliary Movement of Choroid Plexus Epithelial Cells Ex Vivo -
Research • NeuroscienceLive Imaging of the Ependymal Cilia in the Lateral Ventricles of the Mouse Brain -
Research • BiologyInvestigating Flagella-Driven Motility in Escherichia coli by Applying Three Established Techniques in a Series -
Research • BiologyBiophysical Characterization of Flagellar Motor Functions
Mitosis
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Education • Core: Molecular BiologyForces Acting on Chromosomes -
Education • Core: Molecular BiologySpindle Assembly -
Education • Core: Molecular BiologyThe Mitotic Spindle -
Research • BiologyReconstitution of Basic Mitotic Spindles in Spherical Emulsion Droplets -
Education • Core: Molecular BiologyAttachment of Sister Chromatids
Intermediate Filaments
Coordination and Cooperation Between Cytoskeletal Elements
