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Journal
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Biology
/
Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
JoVE Journal
Biology
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JoVE Journal
Biology
Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
DOI:
10.3791/63432-v
•
06:39 min
•
February 04, 2022
•
João Paulo Rodrigues Marques
,
Laura Galvan Nuevo
1
Faculty of Animal Science and Food Engineering, Department of Basic Sciences
,
University of São Paulo
,
2
Laboratory of Nuclear Instrumentation, Center of Nuclear Energy in Agriculture
,
University of São Paulo
Chapters
00:04
Introduction
00:58
Sample Harvesting, Fixation, and Dehydration
01:42
Historesin Embedding Procedure
02:23
Polymerization
03:24
Sectioning and Double Staining Process
04:27
Results: Double-Staining Method to Detect Pectin in Plant-Fungus Interaction
05:59
Conclusion
Summary
Automatic Translation
English (Original)
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Automatic Translation
This protocol describes a microscopical method to detect pectin in coffee-fungus interaction.
Tags
Keywords: Double-staining
Pectin
Plant-fungus Interaction
Histopathological Studies
Coffee Rust
Cercosporiosis
Karnovsky Fixative
Cacodylate Buffer
Glycol Methacrylate
Polymerization
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