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Herstellung transgener Xenopus laevis Durch Restriktionsenzym-vermittelte Integration und Reaktorsicherheit Transplantation
JoVE Journal
Biology
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JoVE Journal Biology
Production of Transgenic Xenopus laevis by Restriction Enzyme Mediated Integration and Nuclear Transplantation
DOI:

09:48 min

August 21, 2010

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Chapters

  • 00:04Title
  • 00:11Introduction
  • 01:21Sperm Nuclei Preparation
  • 03:33Determinining Nuclei Yield
  • 04:15Preparation of High Speed Extract
  • 06:58Transgenesis Reaction and Nuclear Transfer
  • 09:05Representative Transgenic Embryo
  • 09:24Conclusion

Summary

Automatic Translation

Dieses Video-Protokoll beschreibt eine Methode zur Herstellung transgener<em> Xenopus laevis</em> Durch die Einführung von Transgenen in Spermakerne durch nukleare Transplantation in unbefruchteten Eiern gefolgt.

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