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使用丙烯酰胺水凝胶的细胞功能的研究矩阵刚度的影响
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Studying the Effects of Matrix Stiffness on Cellular Function using Acrylamide-based Hydrogels
Please note that all translations are automatically generated.
Click here for the English version.
使用丙烯酰胺水凝胶的细胞功能的研究矩阵刚度的影响
DOI:
10.3791/2089-v
•
10:19 min
•
August 10, 2010
•
Alexandra Cretu
,
Paola Castagnino
,
Richard Assoian
1
Department of Pharmacology
,
University of Pennsylvania
Chapters
00:04
Title
00:49
Introduction
01:10
Coverslip Preparation
03:50
Preparation of Hydrogels
06:15
Modeling
08:35
Representative Results
09:50
Conclustion
Summary
Automatic Translation
English (Original)
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Automatic Translation
可以仿照下层刚度对细胞功能的影响<em>在体外</em>使用不同的符合标准的聚丙烯酰胺凝胶。
Tags
Matrix Stiffness
Cellular Function
Acrylamide-based Hydrogels
Fibrosis
Cancer
Cardiovascular Disease
Cell Biology
ECM-coated Hydrogels
Polyacrylamide
NaOH
3-APTMS
Glutaraldehyde
Acrylamide (AC)
Bis-acrylamide (Bis-AC)
Ammonium Persulfate
N-hydroxysuccinimide (NHS)
Rheology
Atomic Force Microscopy (AFM)
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