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Chapters
- 00:05Title
- 01:51Preparing Worm Samples for Metabolite Quantitation
- 04:37Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
- 07:11Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
- 08:39Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
- 11:40Conclusion
Stabil isotop profilering av gaskromatografi masspektrometrisk analys av mellanhand metabola flux beskrivs i rundmasken,<em> Caenorhabditis elegans</em>. Metoder beskrivs för att bedöma isotopisk anrikning i koldioxid, organiska syror och aminosyror efter isotop exponering antingen under utveckling på agarplattor eller i vuxen ålder i flytande kultur.
Tags
Stable Isotopic ProfilingIntermediary MetabolismMetabolic FluxCaenorhabditis ElegansNematode Growth Media Agar PlatesPharmacologic TreatmentsHigh Performance Liquid Chromatography (HPLC)Mass SpectrometryCarbon DioxideGlycolysisPyruvate MetabolismTricarboxylic Acid CycleIsotope Exposure TimeBacterial Clearing ProtocolsWorm Disruption Methods
