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在开发和成年阶段的中介代谢通量的稳定同位素分析
线虫</em
JoVE Journal
Biology
This content is Free Access.
JoVE Journal
Biology
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage
Caenorhabditis elegans
Please note that all translations are automatically generated.
Click here for the English version.
在开发和成年阶段的中介代谢通量的稳定同位素分析
线虫</em
DOI:
10.3791/2288-v
•
12:10 min
•
February 27, 2011
•
Marni J. Falk
2
,
Meera Rao*
1
,
Julian Ostrovsky*
1
,
Evgueni Daikhin
,
Ilana Nissim
,
Marc Yudkoff
2
1
Department of Pediatrics
,
The Children’s Hospital of Philadelphia
,
2
Department of Pediatrics
,
University of Pennsylvania
Chapters
00:05
Title
01:51
Preparing Worm Samples for Metabolite Quantitation
04:37
Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
07:11
Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
08:39
Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
11:40
Conclusion
Summary
Automatic Translation
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Automatic Translation
稳定同位素分析气相色谱质谱中介代谢通量的质谱分析中所述的线虫,<em>线虫</em>。详细的评估中的二氧化碳,有机酸,氨基酸和同位素暴露在琼脂平板上的发展,或在液体培养成年的同位素富集的方法。
Tags
Stable Isotopic Profiling
Intermediary Metabolism
Metabolic Flux
Caenorhabditis Elegans
Nematode Growth Media Agar Plates
Pharmacologic Treatments
High Performance Liquid Chromatography (HPLC)
Mass Spectrometry
Carbon Dioxide
Glycolysis
Pyruvate Metabolism
Tricarboxylic Acid Cycle
Isotope Exposure Time
Bacterial Clearing Protocols
Worm Disruption Methods
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