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Stabiele Isotopen Profilering van Intermediair metabole flux in het ontwikkelen en volwassen stadium Caenorhabditis elegans</em
JoVE Journal
Biology
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JoVE Journal Biology
Stable Isotopic Profiling of Intermediary Metabolic Flux in Developing and Adult Stage Caenorhabditis elegans
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Stabiele Isotopen Profilering van Intermediair metabole flux in het ontwikkelen en volwassen stadium Caenorhabditis elegans</em

DOI:
10.3791/2288-v

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12:10 min

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February 27, 2011

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, , , , ,
1Department of Pediatrics,The Children’s Hospital of Philadelphia, 2Department of Pediatrics,University of Pennsylvania

Chapters

  • 00:05Title
  • 01:51Preparing Worm Samples for Metabolite Quantitation
  • 04:37Processing Neutralized Samples for Amino Acid and Organic Acid Analysis by GC/MS
  • 07:11Monitoring Isotopic Utilization in Worms on Plates by Tracing Labeled Carbon in Atmospheric Carbon Dioxide
  • 08:39Results of Stable Isotopic Profiling of Intermediary Metabolism and Metabolic Flux
  • 11:40Conclusion

Summary

Automatic Translation

Automatic Translation

Stabiel isotopische profilering door middel van gaschromatografie massaspectrometrische analyse van intermediaire metabole flux wordt beschreven in de nematode,<em> Caenorhabditis elegans</em>. Methoden worden voor het beoordelen van isotopen verrijking in kooldioxide, organische zuren en aminozuren na isotoop blootstelling zowel tijdens de ontwikkeling op agar platen of tijdens de volwassenheid in vloeibare cultuur.

Tags

Stable Isotopic ProfilingIntermediary MetabolismMetabolic FluxCaenorhabditis ElegansNematode Growth Media Agar PlatesPharmacologic TreatmentsHigh Performance Liquid Chromatography (HPLC)Mass SpectrometryCarbon DioxideGlycolysisPyruvate MetabolismTricarboxylic Acid CycleIsotope Exposure TimeBacterial Clearing ProtocolsWorm Disruption Methods

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