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Chapters
- 00:05Title
- 00:57Introduction
- 01:41Making a Pick
- 02:45Picking Worms
- 03:40Ethyl Methane Sulfonate (EMS) Mutagenesis
- 06:33Psoralen Mutagenesis
- 09:27Results of Mutagenesis of P. pacificus
- 10:01Conclusion
Screening for mutants with phenotypic defects is a straightforward method for identifying genes that function in a given biological process. In this article we describe how to culture free living worms (e.g., Pristionchus pacificus) in the laboratory and show two different mutagenesis methods, EMS and TMP/UV.
Tags
Worm LabCulturing WormsMutagenesisNematodesLaboratory MaintenanceEthyl Methane SulfonateEMS45′8-trimethylpsoralenTMP/UVGenetics StudiesSelf-fertilizing HermaphroditesMating SystemAgar PlatesBacterial LawnPoint MutationsSmall DeletionsRecessive MutationsPristionchus PacificusPhenotypesUncoordinated MutantsDumpy MutantsTransformer Mutants
