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In tempo reale immagini di leucotriene B 4 Migrazione cellulare mediata e BLT1 Interazioni con β-arrestina
JoVE Journal
Immunology and Infection
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JoVE Journal Immunology and Infection
Real-time Imaging of Leukotriene B4 Mediated Cell Migration and BLT1 Interactions with β-arrestin
DOI:

13:45 min

December 23, 2010

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Chapters

  • 00:05Title
  • 01:13Introduction
  • 01:48Preparation of Bone Marrow Derived Dendritic Cells (BMDC)
  • 02:42Preparation of Lipid Loaded Micropipette
  • 04:20Live-cell Imaging of Cell Migration in Response to Ligand Application
  • 05:25Representative Results for Migration of BMDCs
  • 06:03Live Cell Imaging of Transfected RBL-2H3 Cells
  • 09:49Image Analysis
  • 10:51Representative Results
  • 13:03Conclusion

Summary

Automatic Translation

Questo documento descrive la metodologia per determinare la risposta chemiotattica dei leucociti ai ligandi specifici e identificare le interazioni tra recettori di superficie della cellula e le proteine ​​citosoliche utilizzando vivere tecniche di imaging cellulare.

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