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Respirometric皂甙通透心脏纤维的氧化磷酸化的评估

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Respirometric Oxidative Phosphorylation Assessment in Saponin-permeabilized Cardiac Fibers

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Respirometric皂甙通透心脏纤维的氧化磷酸化的评估

DOI:

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11:10 min

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February 28, 2011

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Curtis C. Hughey, Dustin S. Hittel², Virginia L. Johnsen, Jane Shearer²

¹Department of Biochemistry and Molecular Biology, Faculty of Medicine,University of Calgary, ²Faculty of Kinesiology,University of Calgary

Chapters

00:05Title
01:16Cardiac Fibers Mechanical Preparation
02:48Cardiac Fiber Bundle Chemical Preparation and Washing
03:58Wet Weight Determination
04:39Respirometric OXPHOS Using Substrate-Inhibitor-Uncoupler Titrations
08:33Representative Results for Respirometric OXPHOS
10:43Conclusion

Summary

Automatic Translation

皂甙与respirometric氧化磷酸化的分析，结合通透的纤维制备提供了线粒体功能的综合评估。线粒体呼吸在生理和病理状态下，可以反映不同监管规定的影响，包括线粒体相互作用，形态和生物化学。

Tags

Respirometric Oxidative Phosphorylation Saponin-permeabilized Cardiac Fibers Mitochondrial Function Energy Metabolism Oxidative Stress Apoptosis Aging Mitochondrial Encephalomyopathies Drug Toxicity Clark Electrode Substrate-inhibitor-uncoupler Titration Protocols Electron Transport Chain Sarcolemma Muscle Fibers Saponin-skinned Cardiac Fibers Oxygen Consumption Measurements

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