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Chapters
- 00:05Title
- 01:16Cardiac Fibers Mechanical Preparation
- 02:48Cardiac Fiber Bundle Chemical Preparation and Washing
- 03:58Wet Weight Determination
- 04:39Respirometric OXPHOS Using Substrate-Inhibitor-Uncoupler Titrations
- 08:33Representative Results for Respirometric OXPHOS
- 10:43Conclusion
Saponine-gepermeabiliseerde vezels voorbereiding in combinatie met respirometrische oxidatieve fosforylering analyse geeft integratieve beoordeling van de mitochondriale functie. Mitochondriale ademhaling in fysiologische en pathologische toestanden kan weerspiegelen verschillende regelgevende invloeden waaronder mitochondriale interacties, morfologie en biochemie.
Tags
Respirometric Oxidative PhosphorylationSaponin-permeabilized Cardiac FibersMitochondrial FunctionEnergy MetabolismOxidative StressApoptosisAgingMitochondrial EncephalomyopathiesDrug ToxicityClark ElectrodeSubstrate-inhibitor-uncoupler Titration ProtocolsElectron Transport ChainSarcolemmaMuscle FibersSaponin-skinned Cardiac FibersOxygen Consumption Measurements
