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Chapters
- 00:05Title
- 01:16Cardiac Fibers Mechanical Preparation
- 02:48Cardiac Fiber Bundle Chemical Preparation and Washing
- 03:58Wet Weight Determination
- 04:39Respirometric OXPHOS Using Substrate-Inhibitor-Uncoupler Titrations
- 08:33Representative Results for Respirometric OXPHOS
- 10:43Conclusion
Saponin-permeabilized fiber förberedelser i samband med respirometric oxidativ fosforylering analys ger integrerad bedömning av mitokondriell funktion. Mitokondriell andning i fysiologiska och patologiska tillstånd kan spegla olika rättsliga influenser inklusive mitokondriella interaktioner, morfologi och biokemi.
Tags
Respirometric Oxidative PhosphorylationSaponin-permeabilized Cardiac FibersMitochondrial FunctionEnergy MetabolismOxidative StressApoptosisAgingMitochondrial EncephalomyopathiesDrug ToxicityClark ElectrodeSubstrate-inhibitor-uncoupler Titration ProtocolsElectron Transport ChainSarcolemmaMuscle FibersSaponin-skinned Cardiac FibersOxygen Consumption Measurements
