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Chapters
- 00:05Title
- 01:09Agrobacterium Transient Infiltration
- 04:04Assessing Results with a Histochemical GUS Assay
- 04:50Assessing Results with a Fluorometric MUG Assay
- 06:34Cell Death as Measured by GUS Expression
- 07:04Conclusion
Der programmierte Zelltod-Assays häufig in Säugetier-Systemen wie DNA laddering oder TUNEL-Assays, sind oft nur schwer in Pflanzen zu reproduzieren. In Kombination mit einem GUS-Reporter-Systems, schlagen wir eine schnelle, auf pflanzlicher Basis transienten Assay, um das Potenzial der Tod Eigenschaften bestimmter Gene zu analysieren.
Tags
A β-glucuronidase (GUS)Transient Plant Expression SystemReporter GenePositive Regulators Of Cell DeathN. Benthamiana LeavesCo-infiltration35S Driven Expression CassetteGene AnalysisGUS Vector PCAMBIA 2301Agrobacterium Strain LBA4404Live CellsMarker GeneAnti-apoptotic GenesPro- And Anti-death PlayersBcl-2 Associated AthanoGene (BAG) FamilyMammalian Inducers Of Cell DeathBAXPost-translational Modification/activation Of ProteinsRapid And Sensitive Plant Based Method
