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活大鼠皮层神经元线粒体膜电位和活性氧的测定
JoVE Journal
Neuroscience
This content is Free Access.
JoVE Journal Neuroscience
Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons

活大鼠皮层神经元线粒体膜电位和活性氧的测定

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09:56 min

May 23, 2011

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09:56 min
May 23, 2011

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Summary

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我们证明在皮层神经元的应用荧光指标,TMRM TMRM荧光强度的相对变化,以确定应用程序之前和之后的一个特定的刺激。我们也应用荧光探针H<sub> 2</sub> DCF - DA评估皮层神经元的活性氧物种的相对水平。

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