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09:56 min
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May 23, 2011
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Wir demonstrieren die Anwendung der Fluoreszenz-Indikator, TMRM, in kortikalen Neuronen, die relativen Veränderungen in TMRM Fluoreszenzintensität vor und nach der Anwendung eines bestimmten Stimulus zu bestimmen. Wir zeigen auch die Anwendung der Fluoreszenz-Sonde H<sub> 2</sub> DCF-DA auf die relative Höhe der reaktiven Sauerstoffspezies in kortikalen Neuronen zu beurteilen.
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Cite this Article
Joshi, D. C., Bakowska, J. C. Determination of Mitochondrial Membrane Potential and Reactive Oxygen Species in Live Rat Cortical Neurons. J. Vis. Exp. (51), e2704, doi:10.3791/2704 (2011).
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