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使用2',7'-二氯荧光素双乙酸探针和流式细胞术在穆勒神经胶质细胞中定量活性氧
 
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使用2',7'-二氯荧光素双乙酸探针和流式细胞术在穆勒神经胶质细胞中定量活性氧

Article DOI: 10.3791/63337-v 14:25 min May 13th, 2022
May 13th, 2022

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在这里,我们提出了一种系统化,可访问和可重复的方案,以使用Müller神经胶质细胞(MGCs)中的2',7'-二氯荧光素二乙酸探针(DCFH-DA)检测细胞活性氧(ROS)。该方法用流式细胞仪量化总细胞ROS水平。该协议非常易于使用,适用且可重复。

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神经科学,第183期,
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