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一个快速高分辨率的荧光成像的方法在半厚的脑片
JoVE Journal
Neuroscience
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JoVE Journal
Neuroscience
A Rapid Approach to High-Resolution Fluorescence Imaging in Semi-Thick Brain Slices
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一个快速高分辨率的荧光成像的方法在半厚的脑片
DOI:
10.3791/2807-v
•
04:35 min
•
July 26, 2011
•
Jennifer Selever
,
Jian-Qiang Kong
,
Benjamin R. Arenkiel
4
1
Department of Molecular & Human Genetics
,
Baylor College of Medicine (BCM)
,
2
Precisionary Instruments Inc.
,
3
Departments of Molecular & Human Genetics and Neuroscience
,
Baylor College of Medicine (BCM)
,
4
Jan and Dan Duncan Neurological Research Institute
,
Texas Children's Hospital
Chapters
00:05
Title
01:01
Agarose Embedding of Fixed Mouse Brain Tissue
01:55
Sectioning the Embedded Brain Tissue Using the Compresstome
02:33
Optical Clearing of the Brain Tissue Sections
03:14
Mounting the Brain Tissue Sections for Imaging
03:53
Epifluorescent and Confocal Microscopy of Intact Thick Brain Slices
04:16
Conclusion
Summary
Automatic Translation
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Automatic Translation
在这里,我们描述了一个快速和简单的的方法,形象荧光标记的细胞在半厚的脑片。通过固定,切片,和光清除脑组织中,我们介绍了如何标准啶或共焦成像可用于个人完整的神经组织内的细胞和神经网络的可视化。
Tags
High-resolution Fluorescence Imaging
Semi-thick Brain Slices
Neuroscience
Neuroanatomical Maps
Molecular Genetics
Light Microscopy
Neuronal Morphologies
Molecular And Cellular Makeup
Transgenic And Gene Targeting Technologies
Fluorescent Proteins
Marine Invertebrates
Cell Type-specific Promoter Activity
Brain Structure And Function
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